Increased numbers of Tregs and decreased numbers of Th1 and Th17 cells in DSSKO mice after zymosan exposure. A, Representative flow plots of Tregs in the LNs of WT, SLAP−/−, SKG, and DSSKO mice 10 wk after zymosan exposure. B, Frequency and absolute number of LN Tregs in each group as shown in A. C, Intracellular staining of IL-17 and IFN-γ in CD4+ cells from the LNs of WT, SLAP−/−, SKG, and DSSKO mice 10 wk after zymosan exposure. D, Frequency and absolute number of IL-17 and IFN-γ expressing CD4+ LN cells from each group as shown in C. E, Ratio of Tregs compared with Th17 or Th1 cells in zymosan-treated mice. Data represent the average of 7 to 10 mice per genotype (±SEM) from three independent experiments.