FIG. 1.

Injection of infected mice leads to a differential increase in viral load and the frequency of latently-infected cells in pups and adult mice. Mice were infected with γHV-68 and at 30 dpi were injected with 10 nmol CpG-ODN or with PBS as a control. Three days following injection, the spleens and lungs were removed. DNA was extracted from a portion, while the rest was processed to make a single-cell suspension. (A) DNA was extracted from whole spleen or lung tissues and viral load was measured by quantitative PCR. (B) The frequency of latently-infected cells that could reactivate virus was measured by limiting dilution assay (*p < 0.05 compared to mock-infected animals; CPE, cytopathic effect).