FIG. 3.

Mice infected as pups or adults have different proportions of B-cell subsets in the spleen following injection with CpG. Eight-day-old mice were infected with γHV-68 and at 30 dpi were injected with 10 nmol CpG-ODN. After 3 d the spleens were removed and put into a single-cell suspension. The cells were stained with either FITC-PNA and PE-anti B220, or PerCP/Cy5.5-CD19, PE/Cy7-CD23, and APC-CD21, to determine the B-cell phenotype. (A) Representative flow cytometry plots of B-cell subsets in mice infected as pups or adults. (B) Percentage of germinal center, follicular zone, and marginal zone B cells and mean fluorescent intensity of PNA on germinal center B cells (control: n = 4, CpG: n = 5; *p < 0.05 compared to mock-injected animals).