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Generation of Cdc14b-deficient mice. (A) Strategy used to generate a conditional allele of Cdc14b. (B) Southern blot analysis. Genomic DNA isolated from tail snips was digested with BamHI, separated on an agarose gel, and blotted. The blot was probed with a 3′ probe shown in panel A. WT, wild type. (C) RT-PCR analysis of the expression of wild-type Cdc14b and Cdc14bΔE2.
