Fig. 4.

Proficient G₂/M DNA damage checkpoint in Cdc14b-deficient MEFs. (A) Fluorescence-activated cell sorting (FACS) analysis of iMEFs 2 h after release from 48-h serum starvation. (B) Mitotic-index analysis of cells without gamma irradiation. Nocodazole (80 ng/ml) was added to the medium of the cells in panel A, and the cells were harvested at 4 and 8 h after the addition of nocodazole (6 and 10 h after release). The error bars indicate standard deviations. (C) Mitotic-index analysis of cells with 10-Gy gamma irradiation. The cells were treated the same as those in panel B but were irradiated immediately before the addition of nocodazole. (D) Western blot analyses of mitotic regulators in the cells from panels B and C. Time zero (0 h) was 2 h after release from serum starvation.