Fig. 4.

Knockdown of MK-STYX protects against intrinsic, but not extrinsic, apoptosis. (A, B, D, E, and F) Control or MK-STYX-specific siRNAs were transfected into HeLa cells for 24 h, and the cells were then plated onto an electrode-containing plate (e-plate), where they adhered for 24 h and were then treated with 100 nM paclitaxel (A), 10 ng/ml actinomycin D (B), 0.5% serum (growth factor deprivation) (D), 10 μg/ml tunicamycin (E), and 10 ng/ml TNF-α cotreated with cycloheximide (F). (C) For UV irradiation, cells were transfected with siRNAs for 24 h before exposure to 80 J/m² of UV irradiation. The cells were immediately collected and plated onto an e-plate, where they were allowed to adhere and proliferate. Cell viability was tracked using the xCelligence real-time viability system. The error bars indicate standard deviations.