Fig. 3.

CCN5 represses activated transcription. (A) Scheme of LexA-GAL4-driven E1B promoter-reporter construct. LexA-VP16 is an activator and GAL4-CCN5 a repressor. (B) Activities of the indicated GAL4-CCN5 fusion proteins relative to the reporter alone in 293T cells. Cells were cotransfected with 0.1 μg of LexA-VP16 alone or in combination with 1 μg of constructs encoding the indicated GAL4-CCN5 fragments and 0.5 μg of GAL4-driven luciferase reporter and 0.1 μg of RSV-β-galactosidase construct as an internal control. Cells were harvested 48 h later. Extracts were assayed for luciferase and β-galactosidase activities. The presence of LexA-VP16 is indicated. The results shown represent the average of three independent experiments assayed in duplicate. Significant differences: *, P < 0.05 versus control.