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. 2011 Jul;138(1):117–130. doi: 10.1085/jgp.201010586

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© 2011 Clausen et al.

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Figure 3. — Effect of elevated [K⁺]_o and salbutamol on force development in mouse soleus. Muscles from 12.5-wk-old WT and mutant mice were mounted in force transducers for isometric contractions in standard KR buffer and stimulated as indicated every 10 min using 2-s trains of 120-Hz pulses of 0.2-ms duration and 12 V. After 60 min, the buffer was changed to a KR in which K⁺ was increased by 6 mM and Na⁺ was decreased by 6 mM to maintain isoosmolarity. After 50 min, 10⁻⁶ M salbutamol was added to all muscles, and stimulation every 10 min continued. Each point represents the mean of observations on four muscles, with error bars denoting SEM.