Table IV.

[³H]Ouabain binding sites in soleus, EDL, and tibialis anterior muscles of WT and mutant mice

Muscle	[3H]Ouabain binding	P-value
	pmol/g wet wt
WT soleus	746 ± 28 (6)	<0.004
Mutant soleus	952 ± 43 (10)
WT EDL	647 ± 14 (4)	<0.001
Mutant EDL	1,047 ± 37 (4)
WT tibialis anterior	417 ± 28 (8)	<0.001
Mutant tibialis anterior	554 ± 22 (8)

[³H]Ouabain binding in 12.5-wk-old WT and mutant mice. Whole frozen soleus and EDL muscles or biopsies of tibialis anterior muscles were incubated for 2 h at 37°C in Tris-vanadate buffer containing 10⁻⁶ M [³H]ouabain. After the incubation, the muscles were washed four times for 30 min in ice-cold Tris-vanadate buffer to remove unbound [³H]ouabain, blotted, and taken for counting and calculation of specific [³H]ouabain binding as described in the Materials and methods section.