Figure 2.
Reduced cytokine production by IkkαAA CD4+ T cells primed in vivo. WT or IkkαAA CD4+ T cells, 107 cells/mouse, were transferred into Rag1−/− recipients through tail vein (n = 3). EAE was induced by immunizing mice with MOG peptide 24 h later. Mice were sacrificed on day 22 after disease onset, and their splenocytes, 0.5 × 106/well, were cultured in complete DME with or without 5–50 µg of MOG peptide. [3H]thymidine was added 48 h after the initiation of the cell culture. Cells were harvested 18 h later, and [3H]thymidine incorporation was measured (a). (b–e) Cytokine concentrations were measured by ELISA 40 h after the initiation of the culture. Results shown are means ± SD of triplicate cultures. The differences between the two groups are statistically significant for all cultures with MOG peptide (P < 0.01). CPM, count per minute. Data are representative of two independent experiments.