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. 2011 Jul 4;208(7):1367–1376. doi: 10.1084/jem.20110278

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© 2011 Shi et al.

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Figure 3. — HIF1α deficiency promotes T_reg cell differentiation and alters T_H17/T_reg cell balance. (A) Naive T cells from WT and HIF1α^−/− mice were activated in the presence of 1 or 10 ng/ml TGF-β for 5 d, followed by Foxp3 staining. Data represent four independent experiments. (B) Naive T cells from WT and HIF1α^−/− mice were activated in the presence of IL-2 and varying amount of TGF-β for 5 d, followed by real-time PCR analyses of Foxp3, Ctla4, and Gpr83 expression. (C) Naive T cells from WT and HIF1α^−/− mice were stimulated with splenic DCs and anti-CD3 for 5 d, and Foxp3 mRNA was analyzed. (D) WT and HIF1α^−/− mice were immunized with MOG and, 9 d later, DLN cells were stimulated with MOG for 5 d, followed by Foxp3 staining. (E) Naive T cells from WT and HIF1α^−/− mice were differentiated under T_H17 conditions with varying doses of IL-6 for 5 d, followed by intracellular staining of IL-17 and Foxp3. Data represent four independent experiments. (F) WT naive T cells were differentiated under T_H17 or T_reg cell–inducing conditions, followed by real-time PCR analyses of glycolytic molecules. Expression levels in naive T cells were set to 1. Data represent two independent experiments. Data represent the mean ± SEM (B and C) or the mean ± SD (F).