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. 2011 Jul 11;194(1):137–153. doi: 10.1083/jcb.201009137

Figure 4.

Figure 4.

Premature accumulation of active Ipl1/Aurora along the central spindle reduces midzone length. (A) CPC is prematurely targeted to the central spindle upon Ipl1 inhibition. Sli15-3GFP localization relative to mCherry-Tub1 during early/mid-anaphase B (spindle <8 µm long) in wild-type and ipl1-as6 cells treated with 40 µM 3-MB-PP1. Bar, 2 µm. (B) Quantification of A; P values determined using the Mann-Whitney U test. (C) sli15-17A prematurely targets CPC to the central spindle. Localization of Sli15-3GFP and sli15-17A-3GFP relative to mCherry-Tub1 during early/mid-anaphase B (spindle <8 µm long). Bar, 2 µm. (D) Quantification of C as described in B. (E) sli15-17A reduces spindle midzone length. Representative images of Ase1-4GFP and Spc42-eqFP in SLI15 and sli15-17A cells during anaphase B. Bar, 2 µm. (F) Quantification of Ase1-4GFP zone lengths from cells represented in E. (G) Ipl1 inhibition alleviates the reduced midzone length caused by sli15-17A. Representative images of Ase1-4GFP relative to mCherry-Tub1 in SLI15 ipl1-as6, sli15-17A ipl1-as6, and sli15-17A IPL1 cells treated with 40 µM 3-MB-PP1. Bar, 2 µm. (H) Quantification of Ase1-4GFP zone lengths from cells represented in G. † and ‡, P values determined using the Mann-Whitney U test. ‡, test was of the null hypothesis that ρ = 0.5.