Figure 3.

MAPK signal pathway activation time course for Schwann cells treated with earthworm extract. Schwann cells were treated with 125 μg/ml earthworm for different time as indicated, and then the cells were harvested and extracted for the western blot analysis. The protein expression of the MAPK signal pathway was determined by western blot (a)–(c). α-Tubulin was used as a loading control. Further to confirm the MMP-9 and MMP2 activity by gelatin zymography (d). Lane 1: FBS as positive marker (P).