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. 2011 Jul 15;22(14):2448–2457. doi: 10.1091/mbc.E09-01-0029

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© 2011 Barnhart et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 4: — MAD2/mad2Δ heterozygotes do not arrest in response to a loss of tension on all mitotic chromosomes. We measured cell cycle progression by Western blotting after release from a G1 arrest in the presence or absence of the MCD1, a component of sister chromatid cohesion. Cells were grown to mid-log phase and arrested in G1 with α-factor, and released into glucose to shut off expression of P_GAL1-MCD1. Western blots against Myc (top) or actin (bottom) were performed (n = 3). Pds1-18xMyc serves as a marker for metaphase, and a decrease in its level indicates progression into anaphase. Wild-type diploids progress through the cell cycle without delay. In the absence of sister chromatid cohesion, wild-type cells display an extended arrest. However, MAD2/mad2Δ and mad2Δ/mad2Δ cells do not arrest in response to a loss of tension on all mitotic chromosomes.