TABLE 3:
Strains used in this study.
| Strain | MAT | Genotype | Source |
|---|---|---|---|
| EBY54 | MATa/MATα | Ptet-CDC20-127@TRP1/Ptet-CDC20-127@TRP1 CDC28-VF::HIS3/CDC28-VF::HIS3 A241p1::LEU2 mad1Δ::HIS3/MAD1 mad2Δ::Kanr/MAD2 | This study |
| EBY63 | MATa/MATα | Ptet-CDC20-127@TRP1/Ptet-CDC20-127@TRP1 CDC28-VF::HIS3/CDC28-VF::HIS3 A241p1::LEU2 mad2Δ::HIS3/MAD2 mad3Δ/MAD3 | This study |
| EBY69 | MATa/MATα | bub2Δ::LEU2/bub2Δ::LEU2 | This study |
| EBY70 | MATa/MATα | bub2Δ::LEU2/bub2Δ::LEU2 mad2Δ::URA3/MAD2 | This study |
| EBY73 | MATa/MATα | mad2Δ::Kanr/MAD2 SUP11::URA3/SUP11::URA3 | This study |
| EBY74 | MATa/MATα | SUP11::URA3/SUP11::URA3 | This study |
| EBY75 | MATa/MATα | mad2Δ::Kanr/mad2Δ::LEU2 SUP11::URA3/SUP11::URA3 | This study |
| EBY76 | MATa/MATα | mad2Δ::Kanr/MAD2 mad1Δ::HIS3/MAD1 SUP11::URA3/SUP11::URA3 | This study |
| MAS381 | MATa/MATα | bub2Δ::LEU2/bub2Δ::LEU2 mad2Δ::URA3/mad2Δ::URA3 | This study |
| RDY473 | MATa/MATα | Ptet-CDC20-127@TRP1/Ptet-CDC20-127@TRP1 CDC28-VF::HIS3/CDC28-VF::HIS3 A241p1::LEU2 | This study |
| RDY353 | MATa/MATα | Ptet-CDC20-127@TRP1/Ptet-CDC20-127@TRP1 CDC28-VF::HIS3/CDC28-VF::HIS3 A241p1::LEU2 mad1Δ::HIS3/MAD1 | This study |
| RDY354 | MATa/MATα | Ptet-CDC20-127@TRP1/Ptet-CDC20-127@TRP1 CDC28-VF::HIS3/CDC28-VF::HIS3 A241p1::LEU2 mad2Δ::Kanr/MAD2 | This study |
| RDY355 | MATa/MATα | Ptet-CDC20-127@TRP1/Ptet-CDC20-127@TRP1 CDC28-VF::HIS3/CDC28-VF::HIS3 A241p1::LEU2 mad3Δ/MAD3 | This study |
| SCSY78 | MATa/MATa | PDS1::18xMYC::LEU2/PDS1::18xMYC::LEU2 | This study |
| SCSY215 | MATa/MATαΔ::URA3 | PDS1::18xMYC::LEU2/PDS1::18xMYC::LEU2 Kanr::PGAL1–3xHA::MCD1/Kanr::PGAL1–3xHA::MCD1 | This study |
| SCSY302 | MATa/MATα | his3Δ1/his3Δ1 leu2Δ0/leu2Δ0 met15Δ0/met15Δ0 ura3Δ0/ura3Δ0 | BY4743, Open Biosystems |
| SCSY345 | MATa/MATαΔ::URA3 | PDS1::18xMYC::LEU2/PDS1::18xMYC::LEU2 Kanr::PGAL1–3xHA::MCD1/Kanr::PGAL1–3xHA::MCD1 MAD2/mad2Δ::HPH | This study |
| SCSY348 | MATa/MATαΔ::URA3 | PDS1::18xMYC::LEU2/PDS1::18xMYC::LEU2 Kanr::PGAL1–3xHA::MCD1/Kanr::PGAL1–3xHA::MCD1 mad2Δ::HPH/mad2Δ::HPH | This study |
| SCSY414 | MATa/MATα | MAD1::TAP::HIS3/mad1Δ::URA3 | This study |
| SCSY415 | MATa/MATα | mad1Δ::HIS3/mad1Δ::URA3 | This study |
| SCSY416 | MATa/MATα | MAD2::TAP::HIS3/mad2Δ::Kanr | This study |
| SCS430 | MATa/MATαΔ::URA3 | PDS1::18xMYC::LEU2/PDS1::18xMYC::LEU2 Kanr::PGAL1–3xHA::MCD1/Kanr::PGAL1–3xHA::MCD1 MAD1/mad1Δ::HIS3 | This study |
| SCSY434 | MATa/MATα | mad2Δ::URA3/mad2Δ::Kanr | This study |
| SCSY436 | MATa/MATα | MAD1::TAP::HIS3/MAD1::TAP::HIS3 | This study |
| SCSY438 | MATa/MATα | MAD2::TAP::HIS3/MAD2::TAP::HIS3 | This study |
| SCSY446 | MATa/MATα | MAD1/mad1Δ::HIS3 MAD2::TAP::HIS3/mad2Δ::Kanr | This study |
| SCSY451 | MATa/MATα | MAD1::TAP::HIS3/mad1Δ::HIS3 MAD2/mad2Δ::Kanr | This study |
| SCSY456 | MATa/MATαΔ::URA3 | PDS1::18xMYC::LEU2/PDS1::18xMYC::LEU2 Kanr::PGAL1–3xHA::MCD1/Kanr::PGAL1–3xHA::MCD1 MAD2/mad2Δ::HPH MAD1/mad1Δ::HIS3 | This study |