TABLE 1:

Thermodynamic parameters for binding of KRIT1 FERM domain, wild type and R452E mutant, to Rap1B bound to GMP-PNP.

	n	Kd (μM)	ΔHº (kcal/mol)	ΔGº (kcal/mol)	TΔSº (kcal/mol)
Wild type	1.0	1.8 ± 0.25	−9.5	−7.9	−1.6
R452E	1.0	67 ± 18.7	−7.9	−5.7	−2.2

K_d = 1/K_a; ΔGº = –RT ln K_a ; ΔG = ΔH – TΔS.