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. 2011 Jul 15;22(14):2520–2531. doi: 10.1091/mbc.E11-03-0271

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© 2011 Dymek et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 1: — The flagella of CSC amiRNA mutants have reduced amounts of both CaM-IP2 and CaM-IP3. Western blots of flagella (8 μg) isolated from wild-type and CSC amiRNA mutants were probed with antibodies that recognize the CSC (CaM-IP2 and CaM–IP3), radial spokes (RSP1), inner dynein arm I1 (IC138), and central apparatus (PF20). CaM-IP2 amiRNA mutants 4A2 and 4D6 and the CaM-IP3 amiRNA mutants 6E6 and 1A3 have reduced amounts of both CaM-IP2 and CaM-IP3. 6E6 is the most efficient knockdown; no CaM-IP3 or CaM-IP2 is detectable by Western analysis. RSP1, IC138, and PF20 are present at near wild-type levels in each amiRNA mutant.