Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2011 Jul 15;22(14):2532–2540. doi: 10.1091/mbc.E11-01-0035

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2011 Nakrieko et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

“ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society of Cell Biology.

PMC Copyright notice

FIGURE 2: — Differentiation of ILK-deficient hair follicle stem cells into various hair follicle cell lineages. (A) The dorsal skin of P50 K15.CrePR1-Ilk^f/+ or K15.CrePR1-Ilk^f/f mice (during the second telogen) was treated daily with topical RU486 for 5 d, harvested during the following anagen phase (P70), and processed for immunofluorescence microscopy using antibodies against SOX9 or LEF1, as indicated. Anti–green fluorescent protein (anti-GFP) antibodies were used to detect YFP. Micrographs in panels e, j, o, and t represent higher-magnification images of the areas indicated by the arrows in panels c, h, m, and r, respectively. Bar, 100 μm (for all panels except e, j, o, and t). (B) The tissues described in (A) were also analyzed for Ki67-associated immunoreactivity. Ki67-expressing cells are indicated by arrows. Apoptotic cells, shown by arrowheads, were identified using TUNEL staining. Nuclear DNA was visualized with Hoescht 33342. Bar, 100 μm.