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. 2011 Jul 15;22(14):2579–2587. doi: 10.1091/mbc.E11-04-0279

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© 2011 Sato et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 3: — Subcellular localization of SNAP-29. (A) Subcellular localization of GFP-SNAP-29 in the intestine. GFP-SNAP-29 localizes to punctate structures in the cytoplasm and on the basolateral (arrows) and apical (arrowheads) PMs. GFP-SNAP-29 signal is also detected in the cytosol. (B–D) mC-SNAP-29 and GFP-tagged markers were coexpressed in the intestine. mC-SNAP-29 colocalizes with GFP-SYN-16 on punctate structures (B). Asterisks indicate autofluorescence of gut granules because they appear in green, red, and blue channels. Puncta of mC-SNAP-29 closely associate both with GFP-RER-1 (C) and GFP-RAB-5 (D). Puncta of mC-SNAP-29 are adjacent to GFP-RME-1, but colocalization is observed only in the minor population (E). An enlarged (2×) image is shown in the inset (B and C). Note: All images were observed in living animals. Bars, 5 μm.