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. 2011 Jul 15;22(14):2579–2587. doi: 10.1091/mbc.E11-04-0279

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© 2011 Sato et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 6: — SNAP-29 binds to various syntaxins in vivo. HEK293T cells were transiently transfected with pCI-3xHA-syn-18a, syx-5, syn-16, syn-1, syn-4, or syx-17 in the presence (+) or absence (−) of CMV10-snap-29 (A) or CMV10-ric-4 (B). After 36 h, cells were subjected to immunoprecipitation (IP) using FLAG M2 agarose beads. The resulting precipitates and total cell lysates were examined by immunoblotting (IB) using anti-FLAG (to SNAP-29 or RIC-4) and anti- HA (to syntaxin families) antibodies.