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Recognition of ECSA-2 by serum antibodies in patients with esophageal SCC. E. coli containing ECSA-2 cDNA expression plasmids was incubated with (+) or without (-) IPTG for 2.5 h, with cell lysates subsequently subjected to Western blot analysis using sera from patient-1 (P#1) and patient-2 (P#2) with esophageal SCC. An arrow indicates the IPTG-induced polypeptide that represents the ECSA-2 cDNA product.
