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s-ECSA-2-Ab levels examined by ELISA. The levels of s-ECSA-2-Ab were measured by ELISA using purified GST-ECSA-2 protein and control GST protein. The box plots display the 25th, 50th, and 75th percentiles. The upper and lower cross bars of the box represent 90th and 10th percentiles, respectively. The position of a cut-off value (Average + 3 SD; 0.31) is shown. Values higher than 90th percentile are shown by dots.
