Table 3.
Serum antibody levels against ECSA proteins or peptides examined by ELISA.
| ECSA-1 | ECSA-2 | ECSA-3 | HCA-26/41 | HCA-57/71 | HCA-81/97 | HCA-74/88 | HCA-85/97 | HCA-88/97 | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| HD | P | HD | P | HD | P | HD | P | HD | P | HD | P | HD | P | HD | P | HD | P | |
| Average | 0.004 | 0.132 | 0.050 | 0.145 | 0.011 | 0.230 | 0.009 | 0.010 | 0.025 | 0.067 | -0.018 | 0.086 | 0.024 | 0.023 | -0.012 | -0.016 | 0.032 | 0.021 |
| SD | 0.030 | 0.387 | 0.087 | 0.483 | 0.087 | 0.656 | 0.037 | 0.015 | 0.071 | 0.160 | 0.061 | 0.189 | 0.015 | 0.013 | 0.061 | 0.105 | 0.031 | 0.043 |
| Positive rate | 2/118 | 31/146 | 1/98 | 11/72 | 1/74 | 11/68 | 1/42 | 2/63 | 1/42 | 6/63 | 0/42 | 14/63 | 1/42 | 0/42 | 0/42 | 1/42 | 0/42 | 0/42 |
| (%) | 1.7 | 21.2 | 1.0 | 15.3 | 1.4 | 16.2 | 2.4 | 3.2 | 2.3 | 9.5 | 0.0 | 22.2 | 2.4 | 0.0 | 0.0 | 2.4 | 0.0 | 0.0 |
| P value (T-test) | 0.0001 | 0.0026 | 0.0079 | 0.8848 | 0.0702 | 0.0001 | 0.6690 | 0.8210 | 0.1738 | |||||||||
Synthetic peptides used were; HCA-26/41, biotin-EGGGGVSPPPGQPPCP; HCA-57/71, biotin-SGREVGGSAPCPASR; HCA-81/97, biotin-ARPPLLGSEEPLCPASR; HCA-74/88, biotin-REVRGASARPPLLGS; HCA-85/97, biotin-PLLGSEEPLCPASR; and HCA-88/97, biotin-SEEPLCPASR. ECSA-1, 2 and 3 were GST-fusion proteins.