Table 1.
Antibodies used for histopathological characterization of BTBR mice
| Antigen | Vendor | Catalog # | Host | Dilution | Retrieval | IHC Method |
|---|---|---|---|---|---|---|
| BrdU | Accurate Chemical (Westbury, NY) |
OBT0030 | Rat IgG | 1:300 | None | Free-floating |
| Roche Applied Science (Indianapolis, IN) |
11170376001 | Mouse IgG1 | 1:500 | None | Free-floating | |
| CNPase | Sigma-Aldrich (St Louis, MO) |
C9743 | Rabbit IgG | 1:400 | Borg | Paraffin |
| Drebrin | Sigma-Aldrich (St Louis, MO) |
D3816 | Rabbit IgG | 1:1300 | None | Paraffin |
| Doublecortin | Santa Cruz Biotechnology (Santa Cruz, CA) |
sc-8066 | Goat IgG | 1:2000 | None | Free-floating |
| GAD65 | Millipore (Temecula, CA) |
AB5082 | Rabbit IgG | 1:500 | None | Paraffin |
| GAD67 | Millipore (Temecula, CA) |
MAB5406 | Mouse IgG2a | 1:1000 1:6000 |
Borg | Paraffin Free-floating |
| GFAP | Dako (Carpinteria, CA) |
Z0334 | Rabbit IgG | 1:5000 | Borg | Paraffin |
| Sigma-Aldrich (St Louis, MO) |
G9269 | Rabbit IgG | 1:300 | None | Free-floating | |
| GluR1 | Millipore (Temecula, CA) |
04-855 | Rabbit IgG | 1:50 | Borg | Paraffin |
| Iba1 | Wako Chemicals USA (Richmond, VA) |
19-19741 | Rabbit IgG | 1:1000 | Borg | Paraffin |
| MAP2 | Sigma-Aldrich (St Louis, MO) |
M4403 | Mouse IgG1 | 1:1000 | Borg | Paraffin |
| MBP | Abcam (Cambridge, MA) |
ab2404 | Rabbit IgG | 1:50 | Borg | Paraffin |
| NeuroD | Santa Cruz Biotechnology (Santa Cruz, CA) |
sc-1086 | Goat IgG | 1:250 | None | Free-floating |
| NeuN | Millipore (Temecula, CA) |
MAB377 | Mouse IgG1 | 1:1000 | None | Free-floating |
| NG2 | Millipore (Temecula, CA) |
AB5320 | Rabbit IgG | 1:1200 | None | Free-floating |
| Parvalbumin | EMD/Calbiochem (Gibbstown, NJ) | PC255L | Rabbit IgG | 1:700 | Borg | Paraffin |
| PSA-NCAM | Abcys (Paris, France) |
AbC0019 | Mouse IgM | 1:5000 | None | Free-floating |
| Gift from Dr. T Seki | None | Mouse IgM | 1:500 | None | Free-floating | |
| PSD-95 | Cell Signaling Technology (Beverly, MA) | 3409 | Rabbit IgG | 1:100 | Borg | Paraffin |
| Synapsin 2 | Sigma-Aldrich (St Louis, MO) |
S2947 | Rabbit IgG | 1:1600 1:30,000 |
None | Paraffin Free-floating |
| Synaptophysin | Abcam (Cambridge, MA) |
52636 | Rabbit IgG | 1:250 | Borg | Paraffin |
| VGluT1 | Synaptic Systems (Goettingen, Germany) |
135303 | Rabbit IgG | 1:900 1:16,000 |
None | Paraffin Free-floating |
IHC = immunohistochemistry; BrdU = 5-bromo-2'-deoxyuridine; CNPase = 2',3'-cyclic nucleotide 3'-phosphodiesterase; GAD65 = glutamic acid decarboxylase 65 kDa isform; GAD67 = glutamic acid decarboxylase 67 kDa isoform; GFAP = glial fibrillary acidic protein; GluR1 = glutamate receptor 1; Iba1 = ionized calcium-binding adaptor molecule 1; MAP2 = microtubule-associated protein 2; MBP = myelin basic protein;; NeuN = neuronal nuclei; PSA-NCAM = poly-sialic acid neural cell adhesion molecule; PSD-95 = postsynaptic density protein of 95 kDa; VGluT1 = vesicular glutamate transporter 1