Table 2.
Results of histopathological changes in BTBR versus B6 mice
| Marker | PFC | STR | ACC | AMG | HPC | Comments |
|---|---|---|---|---|---|---|
| AchE | 0 | 0 | 0 | 0 | 0 | |
| BrdU | N/A | N/A | N/A | N/A | - | |
| + GFAP | N/A | N/A | N/A | N/A | + | |
| + NeuN | N/A | N/A | N/A | N/A | - | |
| CNPase | 0 | 0 | + | 0 | 0 | Focal in CC region |
| Drebrin | 0 | 0 | - | 0 | 0 | |
| Doublecortin | 0 | 0 | 0 | 0 | - | |
| GAD65 | 0 | 0 | - | 0 | 0 | |
| GAD67 | 0 | 0 | - | 0 | 0 | |
| GFAP | 0 | 0 | 0 | 0 | 0 | Misorientation (alv., cing.) |
| GluR1 | 0 | 0 | - | 0 | 0 | |
| Iba1 | 0 | 0 | 0 | 0 | 0 | Misorientation (alv., cing.) |
| MAP2 | 0 | 0 | 0 | 0 | 0 | |
| MBP | 0 | 0 | + | 0 | 0 | Focal in CC region |
| NeuroD | N/A | N/A | N/A | N/A | - | |
| NG2 | 0 | 0 | ++ | 0 | 0 | |
| Parvalbumin | 0 | 0 | - | 0 | 0 | |
| PSA-NCAM | 0 | 0 | 0 | 0 | - | |
| PSD-95 | 0 | 0 | - | 0 | 0 | |
| Synapsin 2 | 0 | 0 | - | 0 | 0 | |
| Synaptophysin | 0 | 0 | - | 0 | 0 | |
| Timm's | 0 | 0 | 0 | 0 | 0 | |
| VGluT1 | 0 | 0 | - | 0 | 0 |
PFC = prefrontal cortex; STR = striatum; ACC = anterior cingulate cortex; AMG = amygdala; HPC = hippocampus; AchE = acetylcholinesterase; BrdU = 5-bromo-2'-deoxyuridine; CNPase = 2',3'-cyclic nucleotide 3'-phosphodiesterase; GAD65 = glutamic acid decarboxylase 65 kDa isoform; GAD67 = glutamic acid decarboxylase 67 kDa isoform; GFAP = glial fibrillary acidic protein; GluR1 = glutamate receptor 1; Iba1 = ionized calcium-binding adaptor molecule 1; MAP2 = microtubule-associated protein 2; MBP = myelin basic protein; N/A: not analyzed; NeuN = neuronal nuclei; PSA-NCAM = poly-sialic acid neural cell adhesion molecule; PSD-95 = postsynaptic density protein of 95 kDa; VGluT1 = vesicular glutamate transporter 1.
Comments refer to alterations probably related to callosal agenesis in the BTBR mice (-) = decreased staining compared with B6; (0) = equal staining compared with B6; (+) = moderately increased staining compared with B6; (++) = strongly increased staining compared with B6.