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. 2011 Jun 9;11:65. doi: 10.1186/1472-6750-11-65

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Copyright ©2011 Phrommao et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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SDS-PAGE and casein-zymography of recombinant AprX-SK37. Samples were electrophoresed on a 10-15% SDS polyacrylamide gel (lane 1 & 2) and 12% native polyacrylamide gel (lane 3 & 4). After electrophoresis, the gel was stained with Coomassie brilliant blue (lanes 1 - 3) or detected for protease activity (lane 4). Lane 1, whole cell lysate obtained from E. coli BL21 harbouring plasmid pET-AprX-SK37; lane 2, purified recombinant AprX-SK37; lane 3, purified recombinant AprX-SK37 run in a native polyacrylamide gel; lane 4, purified recombinant AprX-SK37 in casein-zymogram. Standard molecular weights for SDS-PAGE (lane 1 & 2) are marked by arrows.