Table 1.

Number of changes to reactions used in each E. coli knockout mutant compared with the wild-type (WT)

Gene Knockouts	1	2	3	4	5	6	8
Reactions with zero flux in WT and non-zero flux in mutant	12	12	22	25	25	29	36
Reactions with non-zero flux in WT and zero flux in mutant	9	9	16	21	23	17	24