Table 1.

Oligonucleotide primers used in this study

Gene	Primers	Sequences of primers(5'→3')	Length	Anneal tem
ORF2	Fw	AATCTCGAGGCCACCATGACGTATCCAAGG	717 bp	56°C
	Rv	ATTAGATCTTTATTCATTAAGGGTTAAGTG
ub-linker	fw1	gga gagctcgccaccatgcagatcttcgtg	270 bp	56°C
	rv1	gccgccgccgctgccgccgccgccgctgccgccgccgcctcttcccctcaagcg
linker-ORF2	fw2	ggcggcggcggcagcggcggcggcggcagcggcggcggcATGACGTATCCAAGG	790 bp	56°C
	rv2	ctcgag TTATTCATTAAGGGTTAAGTGGGGGG
U-ORF2	fw1	gga gagctc gccaccatgcagatcttcgtg	1030 bp	56°C
	rv2	ctcgag TTATTCATTAAGGGTTAAGTGGGGGG

Note: Italic denotes restriction enzyme site; underline denotes linker; TTA denotes stop.