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. Author manuscript; available in PMC: 2012 Sep 1.
Published in final edited form as: Anal Biochem. 2011 May 8;416(1):27–38. doi: 10.1016/j.ab.2011.05.003

Figure 6.

Figure 6

Progress curve of dethiobiotin formation. Time course of dethiobiotin produced in a standard assay. Dashed line is the raw data in RFU and the solid line is the same data converted into concentration of dethiobiotin formed using equation 4. The assay contained 320 nM BioD, 50 nM BioA, 800 nM KAPA, 20 nM 6, 185 nM streptavidin, 100 mM Bicine, 5 mM ATP, 50 mM NaHCO3, 1 mM MgCl2, 0.1 mM PLP, 1 mM SAM, 0.0025% Igepal CA630, pH 8.6.