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. 2011 Jul 13;6(7):e21651. doi: 10.1371/journal.pone.0021651

Figure 2. EZH2 depletion leads to cell cycle arrest of colon cancer cell lines.

Figure 2

A Immunoblot analyses of HCT116, DLD1, and LoVo cells showing efficient downregulation of EZH2 expression by RNAi. Cyclin D1 levels are indicated. Tubulin, loading control. B Cell cycle analyses by FACS. Cells were treated with two control siRNAs or with EZH2-targeting siRNAs. Percentages of cells in the G1, S, or G2/M phases of the cell cycle are indicated. C Compilation of cell cycle analyses from three independent experiments. Standard deviations are indicated. Asterisks equal p≤0.05, double asterisks equal p≤0.01, n.s. not significant.