Table 2. Taxonomic affiliation of relatively abundant and distinctive bacterial and eukaryotic SSU rDNA fragments from DGGE gels (Figure 6) and correspondence with sequences from SSU rRNA gene libraries.
Band | First Hit | % identity | Phylogenetic ascription | Corresponding OTU |
BACTERIA | ||||
B1 | cher4_2B_90 | 89 | Bacteria; Cyanobacteria; Nostocales | cyanobacteria_OTU1 |
B2 | cher4_2B_41 | 91 | Bacteria; Betaproteobacteria; Burkholderiales | betaproteobacteria_OTU5 |
B3 | cher4_2B_90 | 88 | Bacteria; Cyanobacteria; Nostocales | cyanobacteria_OTU1 |
B4 | cher4_2B_95 | 100 | Bacteria; Bacteroidetes; Sphingobacteria | bacteroidetes_OTU1 |
B5 | cher2_1B_19 | 94 | Bacteria; Alphaproteobacteria | alphaproteobacteria_OTU5 |
B6 | cher4_2B_90 | 88 | Bacteria; Cyanobacteria; Nostocales | cyanobacteria_OTU1 |
B7 | cher6_1B_26 | 93 | Bacteria; Bacteroidetes; Sphingobacteria | bacteroidetes_OTU3 |
B8 | cher2_1B_2 | 98 | Bacteria; Actinobacteria | actinobacteria_OTU14 |
B9 | cher2_1B_2 | 99 | Bacteria; Actinobacteria | actinobacteria_OTU14 |
EUKARYOTES | ||||
E1 | cher2_1E_137 | 97 | Fungi, Ascomycota | ascomycota_OTU5 |
E2 | cher2_1E_115 | 87 | Viridiplantae; Chlorophyta | chlorophyta_OTU3 |
E3 | cher2_1E_113 | 95 | Fungi, Ascomycota | ascomycota_OTU10 |
E4 | cher2_1E_129 | 95 | Fungi, Ascomycota | ascomycota_OTU7 |
E5 | cher4_1E_142 | 89 | Metazoa; Rotifera | metazoa_OTU2 |