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. 2011 Jul 13;6(7):e22107. doi: 10.1371/journal.pone.0022107

Figure 3. HELZ interacts with the poly(A)-binding protein (PABP).

Figure 3

GST pull-down using glutathione-sepharose beads was conducted by incubating crude HeLa cell lysates with recombinant GST, GST-Paip2106–127 and GST-HELZ1023–1199 (A), GST and the indicated GST-HELZ fragments (D), or crude HEK293 cell lysates with GST and GST-PABP554–636 (C). Hypoxic HeLa cell lysates were incubated with GST or GST-HELZ1023–1199 (B). Eluates were subjected to SDS-PAGE and immunoblotting using the indicated antibodies.