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. 2011 Jul 13;6(7):e22107. doi: 10.1371/journal.pone.0022107

Figure 6. HELZ suppression limits cellular proliferation, ribosomal protein S6 phosphorylation and initiation of translation.

Figure 6

(A) HeLa cells were transfected with the indicated siRNA oligonucleotides and cell numbers were assessed every 24 hours using an automated cell counter. (B) Immunoblot analysis of HeLa cells transfected with two different HELZ siRNA oligonucleotides and harvested at indicated time points after transfection (left panel). The experiment was performed three times independently and the pS6 band intensities were quantified 48 hours after transfection (right panel). (C) Protein levels of HELZ, p-S6 and total S6 in stable shRNA control (shctrl) or shRNA HELZ expressing cells were analyzed by immunoblotting. (D) Representative polysomal profile (n = 3) of HeLa cells transfected with control or HELZ siRNA oligonucleotides.