Figure 5. Epitopic PET-Blot analysis.

Illustrations of comparative PrP^res detection with P4 and SAF84 mAb using a PET-blot analysis. A. The brains of a non inoculated TgOvPrP4 mouse and a PrP KO mouse were used as negative controls and showed no PrP^res deposition. B. The brains of a TgOvPrP4 mouse inoculated with C506M3, 87V or TR316211 or with BSE strains by the i.c. route. PrP^res, observable as dark blue deposits on these membranes, was detectable in each case using SAF84 but not using P4 mAb. PrP^res revealed by this antibody was present in the C506M3 and BSE experiment and not in the second passage experiment of 87V or the TR316211 isolate. In C506M3- just as in BSE-infected brains, P4-labeled PrP^res accumulated in less specific brain areas and in smaller quantities than shorter PrP^res forms detected by the SAF84 prion antibody.