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. Author manuscript; available in PMC: 2013 Mar 1.
Published in final edited form as: Neurobiol Aging. 2011 Apr 11;33(3):621.e17–621.e33. doi: 10.1016/j.neurobiolaging.2011.02.015

Figure 5. Endogenous slice microglia associate with processes of striatal neurons in greater numbers following transfection with mHtt, but appear to do so independently of mhtt aggregation.

Figure 5

(A)Representative images of striatal neurons in slices transfected with mhtt plasmids encoding HttN90Q73-CFP (mHtt; blue) and YFP (yellow) after 5DIV reveal CFP+ aggregates in degenerating neurites (arrows) and the neuronal soma (arrowheads). Representative IB4 staining for microglia (red) at 3DIV (B-C) and 5DIV (D-E) in slice cultures transfected with control plasmids (B and D) or mHtt plasmids (C and E). YFP alone is shown at right (yellow) to display fine processes of transfected neurons and show microglial association with striatal neurites (arrows). (C and E) mHtt aggregates (arrowheads) indicated by CFP fluorescence (white) in mHtt-transfected cultures (scale= 25μm).