Figure 2. Lysine acetylation decreases the thermostability of BCA II.
A) Thermal denaturation of unacetylated BCA II (denoted Ac(0)), partially acetylated BCA II (Ac(~6) and Ac(~9)) and peracetylated BCA II (Ac(18)) measured by differential scanning calorimetry (DSC). Integration of peaks produced melting temperature (Tm) values of 69.3 °C (Ac(0)), 65.7 °C (Ac(~6)), 63.9 °C (Ac(~9)), and 49.8 °C (Ac(18)). B) Plot of Tm of Ac(0), Ac(~6), Ac(~9), Ac(18) versus the average number of modifications.