Panel A shows the heart rate, beats per minute (bpm) in the wild type (WT) FVB and Cav1.2 transgenic mice (TG). The average heart rate of pups from the immunized transgenic (TG) Cav1.2 overexpression was 315±36 bpm (n=90) compared to immunized WT FVB with an average heart rate of 282±32 bpm (n=76). Non-Transgenic (NTG) littermate pups born to the same immunized Cav1.2 overexpression showed sinus bradycardia (268±21 bpm, n=109). Pups from the nonimmunized WT FVB and the nonimmunized Cav1.2 overexpression had similar heart rates (426±41 bpm, n=48 and 445±21 bpm, n=15 respectively). Panel B represents the PR interval in the WT FVB and Cav1.2 TG. The average PR interval in the pups from the nonimmunized WT FVB and the nonimmunized Cav1.2 overexpression was similar 32±8 ms (n=48) and 30±7 ms (n=15), respectively. The pups from the immunized WT FVB had a prolonged mean PR interval of 64±14 ms (n=76). The NTG immunized littermates in the Cav1.2 overexpression were similarly not protected and showed an increase in PR interval to 71±12 ms (n=109). The average PR interval of pups from the immunized TG Cav1.2 overexpression improved to 48±10 ms (n=90, p<0.05 compared to the immunized WT FVB and immunized NTG littermates).