Typical proton-decoupled 13C-spectra for wild type (upper panel) and gdh1Δ (lower panel) strains after 2 hours in [1, 2-13C]-acetate. Characteristic splitting of the singlet resonance(s) for glutamate carbons, due to the presence of the 13C label at neighboring carbon(s), are expanded at the top (s, singlet; d, doublet, dd, doublet of doublet; t, triplet). Note that the gdh1 spectrum has been magnified 7-fold.