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. Author manuscript; available in PMC: 2012 Oct 20.

Published in final edited form as: Microbiol Res. 2011 Jan 15;166(7):521–530. doi: 10.1016/j.micres.2010.10.004

Analysis of glutamate carbon ¹³C signal complexity as a function of time in [1,2-¹³C]-acetate. (A) Example of expanded spectra for carbons 2, 4, and 3 (C2, C4, and C3) from the gdh2Δ strain at 30, 60 and 120 minutes. (B) Intensity of multiplet resonances (d, t, dd) as compared to the singlet peak as a function of time. The intensities of multiplet and singlet peaks from three separate experiments were integrated and calculated as a ratio of multiplet to singlet +/− SEM. Data for carbons 1 and 5 are not included, since the intensity was weak and primarily multiplet (data not shown). *, p < 0.05 as compared to the other two strains for this carbon at the same time point.

Analysis of glutamate carbon ¹³C signal complexity as a function of time in [1,2-¹³C]-acetate. (A) Example of expanded spectra for carbons 2, 4, and 3 (C2, C4, and C3) from the gdh2Δ strain at 30, 60 and 120 minutes. (B) Intensity of multiplet resonances (d, t, dd) as compared to the singlet peak as a function of time. The intensities of multiplet and singlet peaks from three separate experiments were integrated and calculated as a ratio of multiplet to singlet +/− SEM. Data for carbons 1 and 5 are not included, since the intensity was weak and primarily multiplet (data not shown). *, p < 0.05 as compared to the other two strains for this carbon at the same time point.