Table 1.
Straina | Genotype | Doubling Time (minutes)b |
|
---|---|---|---|
YNDex | YNAceRaf | ||
Wild Type (BY4742) | MATα his3 leu2 lys2 ura3 | 127+13 | 254+43 |
gdh1Δ (YOR375c) | MATα his3 leu2 lys2 ura3 gdh1Δ::G418 | 132+11 | NDc |
gdh2Δ (YDL215c) | MATα his3 leu2 lys2 ura3 gdh2Δ::G418 | 132+6 | 232+43 |
gdh3Δ (YAL062w) | MATα his3 leu2 lys2 ura3 gdh3Δ::G418 | 133+6 | 272+33 |
Wild type is followed in parentheses by the strain name; for mutants the strain name used in these studies is followed in parentheses by the designation for the disrupted open reading frame.
Doubling times during logarithmic growth in glucose (YNDex) or acetate plus raffinose media (YNAceRaf); Average of 3–5 separate determinations +/− standard deviation. ND = not detectable
Although the gdh1Δ strain does not divide, no loss of viability is observed after 17–19 hours in this medium.