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. Author manuscript; available in PMC: 2012 Jul 10.
Published in final edited form as: Cancer. 2011 Jan 10;117(13):2926–2938. doi: 10.1002/cncr.25868

Figure 1. HNSCC cell lines require glucose and glutamine for maximal cell population growth.

Figure 1

Cells proliferated in normal growth medium (DMEM) (A) or DMEM lacking either glutamine (B) or glucose (C). At indicated times the total DNA content of each well was assayed using a commercially available kit. Y- axis values are expressed as change in cell number at T= Xhr compared to T= 0hr. D) Cells were grown for 72hr in media lacking glucose or glutamine, supplemented with pyruvate. Live/dead cells were evaluated using Trypan blue exclusion. * indicates p<0.05. E) Cells were grown for 48hr in media lacking glucose or glutamine. Cells were collected and subjected to Annexin V PE/7AAD analysis in order to determine the early and late apoptotic as well as the necrotic fraction. Data are representative of multiple independent experiments.