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. Author manuscript; available in PMC: 2012 Jul 10.
Published in final edited form as: Cancer. 2011 Jan 10;117(13):2926–2938. doi: 10.1002/cncr.25868

Figure 8. Loss of WTp53 abrogates glucose starvation induced G1 arrest and sensitizes HN30 cells.

Figure 8

(A) Stable knockdown of WT (HN30shp53) was created. Stimulation with 5-FU (5ng/ml) for 24hr up-regulated expression of WTp53 in HN30 parental and HN30 lentivirus (HN30L) transfected cells. (B) Cells were grown in the presence or absence of glucose for 72hr. Total cell number was assayed at the end of the experimental period. (C) Cells were exposed to 2-DG [5mM] for 72hr. Total cell number was assayed at the end of the experimental period. (D) Cells were glucose starved for 36hr prior to cell cycle analysis using propidium iodide.