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. Author manuscript; available in PMC: 2012 Aug 1.
Published in final edited form as: Antiviral Res. 2011 May 12;91(2):120–132. doi: 10.1016/j.antiviral.2011.05.003

Figure 2. Rebound and clearance assay.

Figure 2

Replicon cells were treated with PSI-353661 for 2 weeks in the absence of G418. Fresh compound was added at every passage. After 2 weeks, cells were passaged into culture medium containing 0.25 mg/mL G418 without inhibitor and cultured for an additional 2 weeks without passaging. An aliquot of cells was harvested at each passage for replicon RNA analysis. Remaining cells were stained with crystal violet at the end of the experiment. (A) Effect of PSI-353661 on the level of HCV replicon RNA in the presence of 0 (□), 8 nM (▲), 20 nM (■), 40 nM (◆) and 80 nM (▲) PSI-353661. Values for the level of HCV RNA are the average of two separate assays. (B) Colony formation in the presence of PSI-353661.