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. 2011 Jul 15;89(1):44–55. doi: 10.1016/j.ajhg.2011.05.023

Figure 3.

Figure 3

Specc1l-GFP Stabilizes Acetylated α-Tubulin-Containing Microtubules

(A) GFP-tagged Specc1l (green) in U2OS cells stabilizes a subset of microtubules (arrows) stained with β-tubulin (red).

(B) The stabilized subset colocalizes with acetylated α-tubulin-containing microtubules (yellow, arrows).

(C) Specc1l-GFP distorts the actin cytoskeleton (red) and cell shape (arrow).

(D–F) In contrast to wild-type Specc1l-GFP (A and D) and Thr190Met-GFP (E), which show no apparent difference in microtubule stabilization, a C-terminal truncation (ΔCHD) completely abolishes stabilization (F).

(G) Notably, the Gln415Pro-GFP mutant significantly reduces stabilization of acetylated α-tubulin-containing microtubules. Scale bars represent 10 μm.