Figure 4.

SPECC1L Colocalizes with Tubulin and Actin

(A and B) Endogenous SPECC1L shows a microtubule-type cytoplasmic expression pattern (arrows); a site of intranuclear expression is also detected (arrowheads; also see [E] below).

(C and D) SPECC1L colocalizes with acetylated α-tubulin (yellow and orange) in the mitotic spindle during cytokinesis (C) and in gap junctions (D).

(E and F) Punctate expression of SPECC1L is seen in the presumptive microtubule-organizing center (MTOC) surrounding centrioles stained with γ-tubulin.

(G and H) Colocalization with F-actin (G, arrows) is better visualized after nocodazole treatment (H, arrows).

(I) After ionomycin treatment, SPECC1L relocates with F-actin to the cell membrane. Scale bars represent 10 μm.