TABLE 2.
ANTIBODIES USED FOR FLOW-CYTOMETRY AND TISSUE HISTOLOGY
| Antigen | Host | Titer | Source |
|---|---|---|---|
| CCSP | Rabbit polyclonal | 1:100 (FC) | In house |
| CCSP | Rabbit polyclonal | 1:10,000 (IF) | In house |
| proSP-C | Rabbit polyclonal | 1:3,000 (IF) | Kind gift from P.Y. Di |
| BrdU | Mouse | 1:100 (FC) | BD Biosciences (San Jose, CA) |
| GFP | Chicken polyclonal | 1:4,000 (IF) | Abcam (Cambridge, MA) |
| Cytokeratin 18 | Mouse monoclonal IgG1, clone # c-04 | 1:500 (IF) | Abcam |
| Sca-1 | Rat IgG2A, clone D7 | 1:200 (FC-Biolegend) | Biolegend (San Diego, CA) |
| EpCAM (PE-Cy7) | Rat IgG2A, clone G8.8 | 1:200 (FC), | Biolegend |
| CD45 (biotinylated) | Rat IgG2B, clone 30-F11 | 1:200 (FC-eBioscience) | eBioscience (San Diego, CA) |
| CD31 (biotinylated) | Rat IgG2A, clone 390 | 2.5:100 (FC) | eBioscience |
| CD34 (biotinylated) | Rat IgG2A, clone RAM34 | 6.5:100 (FC) | eBioscience |
Definition of abbreviations: BrdU = 5-bromo-2′-deoxyuridine; CCSP = Clara cell secretory protein; FC = flow cytometry; GFP = green fluorescent protein; IF = immunofluorescence.