TABLE 2.
QUANTITATIVE REAL-TIME PCR ANALYSIS OF HYPOXIA-INDUCIBLE GENES
| Gene (RQ) | Alum/OVA PBS | Alum/OVA GSNO | OVA/OVA PBS | OVA/OVA GSNO |
|---|---|---|---|---|
| PAI-1 | 1.0 ± 0.13 | 0.77 ± 0.16 | 0.66 ± 0.1 | 0.90 ± 0.13 |
| LOX | 1.0 ± 0.09 | 0.88 ± 0.14 | 1.17 ± 0.08 | 1.47 ± 0.23 |
| VEGF | 1.0 ± 0.04 | 0.65 ± 0.08* | 0.42 ± 0.02* | 0.35 ± 0.04* |
| CD-73 | 1.0 ± 0.07 | 1.27 ± 0.19 | 0.59 ± 0.09* | 0.69 ± 0.1* |
| HIF-2α | 1.0 ± 0.07 | 1.17 ± 0.22 | 0.51 ± 0.11* | 0.40 ± 0.08* |
Definition of abbreviations: Alum, aluminum hydroxide; GSNO, S-nitrosoglutathione; OVA, ovalbumin; RQ, relative quantity.
Quantitative real-time PCR was performed on cDNA synthesized from whole mouse lung RNA and evaluated for the expression levels of genes regulated by hypoxia-inducible factor–1.
*
P < 0.05 compared to Alum/OVA PBS group using ANOVA (n = 8).