TABLE 3.

QUANTITATIVE REAL-TIME PCR ANALYSIS OF IL-13 AND MUCUS METAPLASIA MARKERS, MUC5AC AND GOB5

Gene (RQ)	B6 Alum/OVA	NOS2−/− Alum/OVA	B6 OVA/OVA	NOS2−/− OVA/OVA
IL-13	1.0 ± 0.42	2.49 ± 0.67	10.0 ± 1.37*	18.0 ± 13.9*
MUC5ac	1.0 ± 0.23	2.91 ± 0.15	81.13 ± 47.64*	110.03 ± 23.60*
GOB5	1.0 ± 0.54	0.91 ± 0.26	878.78 ± 342.68*	1699.0 ± 433.74*

Definition of abbreviations: Alum, aluminum hydroxide; NOS2, nitric oxide synthase–2; OVA, ovalbumin; RQ, relative quantity.

Quantitative real-time PCR was performed on cDNA synthesized from whole mouse lung RNA and evaluated for the expression levels of IL-13 or the mucus metaplasia markers, MUC5ac and GOB5.

^*P < 0.05 compared to corresponding Alum/OVA group using ANOVA (n = 5).