Figure 4.

PI3K knockdown inhibited Dilong extract-induced survival and proliferation. Schwann cell was transiently transfected with 100 nM PI3K siRNA for 8 h before Dilong extract treatment. After incubation with 125 μg ml⁻¹ Dilong extract for 24 h, cells were harvested and analyzed by immunoblotting using antibodies against anti-PI3K antibody and Bcl₂. (a) α-tubulin was used as a loading control. Cell cycle distribution was analyzed by flow cytometry. (b) Data (percentage of cells in the indicated phases) are the means ± SD of three independent measurements. ^#compared to control group, P = .066; *compared to Dilong-treated group, P = .037.