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. 2011 May 12;156(3):1464–1480. doi: 10.1104/pp.111.178442

Figure 2.

Figure 2.

Structural properties of visually presymptomatic pp2a-b′γ leaves. A and B, Ultrastructural properties of mesophyll cells in pp2a-b′γ and wild-type (WT Col) plants. The electron micrographs are representative images of palisade mesophyll and spongy mesophyll tissues. Bars = 2 μm. C, Electron micrograph depicting abnormalities in the spongy mesophyll tissue of pp2a-b′γ leaves. Wrinkled plasma membrane indicative of the initiation of cell death and a homogenous extrusion of chloroplast are indicated with the solid and dashed arrow, respectively. C, Chloroplast; M, mitochondrion. Bar = 2 μm. D, Confocal microscopy images of propidium iodide-stained leaf tissues demonstrating the onset of cell death in pp2a-b′γ leaves. Bars = 47.62 μm. E, Blue native gel demonstrating a similar pattern of photosynthetic thylakoid protein complexes in wild-type and pp2a-b′γ leaves. D, Dimer; LHCII, light-harvesting antenna of PSII; M, monomer; SC, supercomplex; T, trimer.